Isolation and Characterization of Microsatellite Markers for Hypochaeris incana (Asteraceae) and Close Relatives

Premise of the study: We developed microsatellite markers to study clonal growth and interspecific hybridization in the Patagonian and subantarctic plant Hypochaeris incana (Asteraceae) and its closest relatives. Methods and Results: We developed primers for microsatellite loci from 454 sequence reads of genomic DNA of H. incana. We tested them on individuals of H. acaulis, H. hookeri, H. incana, H. palustris, and H. tenuifolia. We selected 15 polymorphic microsatellite loci, which delivered clearly scorable fragments in most or all species. With mean values between 0.7 and 0.8, the expected heterozygosity in populations of H. incana is high. Conclusions: Due to high levels of polymorphism, the developed markers make it possible to distinguish between genets and ramets in H. incana. In some markers, null alleles complicate the scoring of genotypes in tetraploids. All of the developed markers are suitable to study interspecific hybridization among this group of closely related species.Fil: Wang, Ping. University of Natural Resources and Life Sciences; AustriaFil: Tremetsberger, Karin. University of Natural Resources and Life Sciences; AustriaFil: Urtubey, Estrella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; ArgentinaFil: Bernhardt, Karl-Georg. University of Natural Resources and Life Sciences; Austri

Stiffening and unfolding of early deposited-fibronectin increase proangiogenic factor secretion by breast cancer-associated stromal cells.

Fibronectin (Fn) forms a fibrillar network that controls cell behavior in both physiological and diseased conditions including cancer. Indeed, breast cancer-associated stromal cells not only increase the quantity of deposited Fn but also modify its conformation. However, (i) the interplay between mechanical and conformational properties of early tumor-associated Fn networks and (ii) its effect on tumor vascularization remain unclear. Here, we first used the Surface Forces Apparatus to reveal that 3T3-L1 preadipocytes exposed to tumor-secreted factors generate a stiffer Fn matrix relative to control cells. We then show that this early matrix stiffening correlates with increased molecular unfolding in Fn fibers, as determined by Förster Resonance Energy Transfer. Finally, we assessed the resulting changes in adhesion and proangiogenic factor (VEGF) secretion of newly seeded 3T3-L1s, and we examined altered integrin specificity as a potential mechanism of modified cell-matrix interactions through integrin blockers. Our data indicate that tumor-conditioned Fn decreases adhesion while enhancing VEGF secretion by preadipocytes, and that an integrin switch is responsible for such changes. Collectively, our findings suggest that simultaneous stiffening and unfolding of initially deposited tumor-conditioned Fn alters both adhesion and proangiogenic behavior of surrounding stromal cells, likely promoting vascularization and growth of the breast tumor. This work enhances our knowledge of cell - Fn matrix interactions that may be exploited for other biomaterials-based applications, including advanced tissue engineering approaches

Three-dimensionally confined diluted magnetic semiconductor clusters. Zn1−xMnxS

We report the first example of a dilute magnetic semiconductor (DMS) confined in all three dimensions (DMS quantum dot). Zn0.93Mn0.07S clusters of not, vert, similar 25 Å diameter are successfully synthesized inside a glass matrix and fully characterized by chemical analysis, x-ray diffraction, extended x-ray absorption fine structure (EXAFS), and photoluminescence spectroscopy. Effect of size quantization on the exciton energy has been observed. Preliminary magnetic susceptibility data are presented and discussed

Democratizing the Courts: How an Amicus Brief Helped Organize the Asian American Community to Support Marriage Equality

In this essay, the authors offer an alternative rationale for amicus practice. This rationale emerges from thier experience working on a brief in support of marriage equality that sixty-three Asian American organizations endorsed. They found that an amicus brief can be an effective tool to engage and educate community-based organizations and their constituencies, thereby helping to advance social justice issues. Their story also illustrates how amicus practice can be used to organize communities around a legal issue and to democratize the courts. In this way, even if the effect of amicus briefs on litigation outcomes may be marginal, the process of creating an amicus brief can have a very powerful impact

The Implications of Nesting in California Redistricting

Evaluates the process of nesting -- aggregating or dividing assembly and senate districts -- on redistricting outcomes, including compactness, minimizing city and county divisions, and the creation of majority minority seats